Reference fibroblast cultures

Table 3.2.7 Mass assignment and reference range for acylcarnitine species accumulating in fibroblast cultures following incubation with palmitate, liCa-valine, liC>>-isoleucine, and L-carnitine...

The lung fibroblast as influenced by both granular and fibrous dust has been studies in vitro by morphological, biochemical, and biophysical methods. In rabbit lung fibroblast cultures, Rhodesian chrysotile, anthophyllite, amosite and crocidolite UICC reference dusts at a lower dose favoured a fi-... 

Portion of an endocytic vacuole forming in the plasma membrane of a cultured fibroblast. The view is from the inside of the cell and shows a large clathrin cage assembling to form a coated vesicle. The overall diameter of the vacuole is 0.2 pm. Clathrin cages vary in size and in the number of faces but are typically 0.1 pm in diameter (see Fig. 8-27 and associated references). Courtesy of Barbara Pearse. 

More general information about the potential role of apoE in cellular lipoprotein uptake has come from studies with cultured cells (e.g., fibroblasts). Such cells manifest surface receptors for LDL that bind apoB, the protein component of LDL. This is followed by receptor-mediated endocytosis, fusion of the endo-cytic vesicles with lysosomes, and LDL degradation within the lysosomes (see Goldstein and Brown, 1979 Brown et al., 1981, for reviews and references). Cholesteryl esters taken into cells in this manner are hydrolyzed by a lysosomal acid lipase. The liberated cholesterol then leaves the lysosome and is used in the cell for membrane synthesis and as a regulator of intracellular cholesterol homeostasis. 

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