Reduction with Clostridium thermoaceticum

The anaerobic bacterium Clostridium thermoaceticum obtains its energy for growth by reduction of C02 with hydrogen (Eq. 16-46). One of the C02 molecules is reduced to formate which is converted via 5-methyl-THF to the methyl corrinoid 5-methoxybenzimidazolyl-... 

Parekh SR, Cher) n M (1994b) High concentrations of acetate with a mutant strain of Clostridium thermoaceticum. Biotechnol Lett 16 139—142 Park DH, Zeikus GK (1999) Utilization of electrically reduced neutral red by Actinobacillus succinogenes physiological function of neutral red in membrane-driven fumarate reduction and energy conservation. J Bacteriol 181 2403-2410... 

The reduction of 2,4-diamino-6-nitrotoluene was therefore investigated with hydrogenase purified from Clostridium pasteurianum and with carbon monoxide dehydrogenase partially purified from Clostridium thermoaceticum (39). In contrast to methods described in earlier publications, a test system was applied by which the nitro-reducing activity could be directly measured the consumption of DANT was assayed spectrophotometrically at 325 nm. 

The reduction of DANT is catalyzed by purified hydrogenase from Clostridium pasteurianum and also by partially purified CO-dehydrogenase from Clostridium thermoaceticum in combination with methyl viologen and/or ferredoxin (39). During the whole... 

In Figure 8 the clostridial pathway of the reduction of 2,4-diamino-6-nitrotoluene to triaminotoluene with the intermediates that are known so far is depicted. Since whole cells of Clostridium pasteurianum and Clostridium thermoaceticum performed the complete reduction to triaminotoluene (39), other enzyme systems that are not yet known must be involved in the reduction of 2,4-DAHAT. 

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