Red blood cell ghosts

Kaplan, J.H., Forbush III, B., Hoffman, J.F. (1978). Rapid photolytic release of adenosine 5 -triphos-phate from a protected analogue Utilization by the Na K pump of human red blood cell ghosts. Biochemistry 10.4617-4624. 

P. D Orazlo and G.A. Rechnitz, Ion electrode measurements of complement and antibody levels using marker-loaded sheep red blood cell ghosts. Anal. Chem. 49, 2083-2086 (1977). 

To gain insight into the effect of physical state and/or molecular organization on lipid oxidation, a variety of model systems have been used. These include dispersions, liposomes or vesicles (37,38), monolayers adsorbed on silica (39,40,41), and red blood cell ghosts (42). In most of these studies, oxidation was conducted at relatively low temperatures, i.e., 20 - 40°C. Very little information is available on the effects of physical state on high temperature oxidative reactions or interactions of lipids. 

Serum is the sample of choice. Enzyme activity in serum is stable for several weeks if the specimen is stored under refrigeration, and for several years if stored at 20 °C. Moderate hemolysis does not interfere if separated serum has been centrifuged to remove red blood cell ghosts. 

Kaplan, J. H., Forbush, B., Hoffman, J. F., Rapid Photolytic Release of Adenosine 5 Triphosphate from a Protected Analog Utilization by Na K Pump of Human Red Blood cell Ghosts, Biochemistry 1978, 17, 1929 1935. 

Arricta et at. (2003) produced a preparation from bovine red blood cell ghosts as a standard for AChE a.ssays. The activity of this standard was stable at —70°C for up to 3 years. 

Arrieta, D,. Ramirez, A., DePciers, E.. Bosworth, D., and Wilson, B. W. (2003), Bovine red blood cell ghost cholinestera.se as a monitoring standard. Bull. Environ. Contam. Toxicol. 71,447-4,52,... 

Intracorporeal applications are far more complex the enzyme should be directed to its target within the patient s body and avoid the immune response. Immobilization to biocompatible supports may reduce the immune response significantly (Klein and Langer 1986). Several systems for enzyme delivery have been envisaged microencapsulation, liposome entrapment (Chen and Wang 1998 Fonseca et al. 2003), microencapsulation (Dai et al. 2005) and artificial red blood cell ghosts (Serafini et al. 2004). An updated review on the subject has been published... 

Red blood cell ghost Uptake Rwsthetic effect 0.96 C5-C10 0.60 1.30 (W)... 

THE INTERACTION OF CHARGED MEMBRANES WITH SURROUNDING IONS AND ION INDUCED SHAPE AND VOLUME CHANGES OF RED BLOOD CELL GHOSTS... 

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