Strategies reconstitution

Paulmurugan R, Umezawa Y, Gambhir SS. Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc Natl Acad Sci USA 2002 99(24) 15608-15613. 

In the first specific site labeling of the baccatin III part of the paclitaxel molecule, a reconstitution strategy was used. Oxidation of baccatin III (17) (Figure 2.16), obtained by hydrolytic cleavage of paclitaxel, to its 13-keto analog, protection of its 7-OH group (18)... 

Figure 2.16 Use of a reconstitution strategy for baccatin Ill-labeling of paclitaxel...

Finally, in a demonstration that a reconstitution strategy can also be used to prepare a labeled analog of the unlabeled starting material, [ C6]BMS-275183 (24) was synthesized as shown in Figure 2.18. 

Figure 2.18 Application of a reconstitution strategy to the synthesis ofpaclitaxel analog f Cei BMS-275183 (24)...

Figure 10.45 Alternative bishydroxylation methods for the use in the oxidative cleavage of diols in the reconstitution strategy...

The synthesis of [23- C]avermectin B la 1183) is a highly instructive example demonstrating the utility of the reconstitution strategy for the carbon-14 labeling of complex molecules. In this case the structurally closely related avermectin B2a (176, R, = H) was... 

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