Recombinase receptor

Feil, R., Wagner, J., Metzger, D., and Chambon, P. (1997) Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains. Biochem. Biophys. Res. Commun. 237, 752-757. 

Fig. 16.6. Single cell activation of a Cre-recombinase fused with the ERT receptor upon two-photon release of its caged hgand. The transgenic embryo expresses a GFP gene flanked by two loxP sites. When these are excised, a dsRed gene is expressed in the target cell and its descendents, the red cells, in the eye of this embryo shown at two different magnifications...

More recently, a model that lacks functional T and B cells (SCID mice) has been described. SCID mice have defective DNA recombinase that results in an inability to make functional antigen receptors (Kotloff et al., 1993 Lpvik, 1995). As shown in Table 10.1-5, SCID mice have been used in a variety of test systems where a profound immunodeficiency is required. 

D. Metzger, A chimeric Cre recombinase inducible by synthetic,but not by natural ligands of the glucocorticoid receptor, Nucleic Acids Res. 1998, 26,4086-4090. 

LTP has been demonstrated experimentally, but does it really influence memory Evidence that it does has been provided by clever experiments with transgenic mice. Using the Cre recombinase (Qiapter 27) the NR2B subunit of the NMDA receptor was overexpressed in the hippocampus and in the forebrain of mice. ° This was expected to provide better synaptic strengthening than for receptors with the similar NR2A subunit. It was found experimentally that these transgenic mice were more intelligent than normal... 

---