Recombinant fructose 1,6-diphosphate aldolase

Von derOsten, C.H., Sinskey, A.J., Barbas El, C.F., Pederson, R.L., Wang, Y.F. and Wong, C.H., Use of a recombinant bacterial fructose-1,6-diphosphate aldolase in aldol reactions preparative syntheses of 1-deoxynojirimycin, 1-deoxymannojirimycin, l,4-dideoxy-l,4-imino-D-arahinitol, and fagomine. J. Am. Chem. Soc., 1989, 111, 3924. 

This chapter describes recent developments in carbohydrate synthesis using microbial aldolases. Recombinant type II fructose-1,6> diphosphate aldolase and type I 2-deoxyribose-5-phosphate aldolase have been exploited for the synthesis of monosaccharides and analogs. A new procedure for the synthesis of dihydroxy acetone phosphate has been developed. Sialic acid aldolase has been used in conjunction with other enzyme-catalyzed modifications of monosaccharides for the synthesis of sialic acid-related sugars. 

---