RECOMBINANT-DNA METHODOLOGY

Wu R, Grossman L, Moldave K, eds. Recombinant DNA Methodology. New York Academic Press, 1989. 

Recombinant DNA methodology has come of age. It has spawned a growing industry that seeks to commercialize products derived from this technology. Most notable among these biotechnology products are those for human therapeutic use, and of these there are two categories proteins and nucleic acids. The research, development, and commercialization of therapeutic proteins derived from biotechnology is far more advanced than that of nucleic... 

Zoller, M.J. (1992) New recombinant DNA methodology for protein engineering. Curr. 

Table 22.2. DNA Polymerases and Their Uses in Recombinant DNA Methodologies...

Retroviruses are RNA-DNA viruses that require host-cell genome integration to complete their replication cycle. The retroviral enzyme reverse transcriptase is an RNA-dependent DNA polymerase that has important uses in recombinant DNA methodologies such as the synthesis of complementary DNA (cDNA). The human immunodeficiency virus (HIV) is the etiologic agent that causes acquired immunodeficiency syndrome (AIDS) in humans. HIV infects and destroys human T cells, which are required for immune system functions. 

HOST CELL In recombinant DNA methodology, a host cell is used for CLONING the recombinant DNA molecule. 

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