Recognition of synergistic intramolecular interaction Phage versus synthetic combinatorial libraries

Recognition of synergistic intramolecular interaction Phage versus synthetic combinatorial libraries 

To identify an individual enriched ligand from synthetic chemical libraries (see the chapter by Zhao and Lam), the number of variants used is limited by the amount of material which is needed for sequence analysis. The use of smaller beads has been made possible by inclusion of an encryption tag, e.g. an oligonucleotide, the sequence of which can be determined after PCR [44,45], The size of such banks is, all the same, similar to phage-display libraries with some 10s molecular species per ml, using 10 pm beads [46], 

CONSENSUS SEQUENCES FOR HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (PSTI) VARIANTS, SELECTED BY PHAGE DISPLAY IN THE pSKAN8 VECTOR, WHICH INHIBIT EITHER BOVINE CHYMOTRYPSIN (CHY) OR HUMAN LEUKOCYTE ELASTASE (HLE) WITH SUBNANOMOLAR K, VALUES (REF. 72 AND P. ROTTGEN AND J. COLLINS, UNPUBLISHED DATA) 

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