Receptor-ligand interaction screening

Figure 2 Decoding of active peptide sequence by activity screening of pools of sublibraries in competition assays. The ability of sublibraries to interfere with a receptor/ligand interaction is measured in a competition assay using ELISA microplates coated with the target receptor. Identification of the sublibrary with the highest inhibitory activity will lead to the characterization of the first residue responsible for activity. Resynthesis of sublibraries with the known first residue and subsequent screening of their activity will lead to the identification of the second residue responsible for activity. The process is repeated until the full sequence is decoded. w...

Fig. 3.1 Steps involved in drug discovery approach - a typical example. Joint efforts of classical genomics, proteomics, homology modelling, receptor- or structure-based screening approaches, and finally the structural biology efforts to determine the 3D structures of target reeeptor and receptor-ligand complex to get structural insight info their interactions...

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