Receptor/ligand assays

Krey, G., Braissant, O., L Horset, E., Kalkhoven, E., Perroud, M., Parker, M. G., and Wahli, W. Patty acids, eicosanoids, and hypolipidemic agents identified as ligands of peroxisome proliferator-activated receptors by coactivator-dependent receptor ligand assay. Mol Endocrinol 11 (1997) 779-791. 

The application of environmentally sensitive chelates have been applied to develop a more straightforward approach to the development of a competitive homogeneous assay. This principle has been applied to some steroid glucuronides (22,23), thyroxine (21) and receptor ligand assays. 

The application of this technology for receptor ligand assays has also increased over the past 2 years. The principles have largely been based upon a solid phase separation through immobilisation of the receptor onto a microtitration plate by either passive coating, specific chemical linkage or by antibody capture (31,32). More recently the use of microtitration filter plates have been adopted for separation based receptor assays. These (Millipore MultiScreen ) are used mainly in conjunction with the fluorescent chelates. 

Several problems limit the interpretation of sTNF-R and IL-lra levels, including the specificity of currently available assays for free receptor or receptor-ligand complexes (El3). In addition, acute or chronic organ dysfunction may significantly alter the clearance of both sTNF-R and IL-lra, and the potential impact of established immunological dysfunction upon their production has not been clarified. 

Harris, A., Cox, S., Bums, D., and Norey, C., Miniaturization of fluorescence polarization receptor-binding assays using CyDye-labeled ligands, /. Biomol. Screen., 8,410,2003. 

Wang, J.-X., Yamamura, H. I., Wang, W, Roeske, W. R. The use of the filtration technique in in vitro radioligand binding assays for membrane-bound and solubilized receptors, in Receptor-Ligand Interactions. A Practical Approach, ed. Hulme, E. C., IRL Press, Oxford,... 

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