Recent advances in nucleic acid amplification and detection

5 Recent advances in nucleic acid amplification and detection 

Many nucleic acid detection strategies use target amplification, signal amplification or both. Invader, branched DNA (bDNA) and rolling circle amplification (RCA) are three approaches. 

The linear RCA method can use both target and signal amplification. A DNA circle (such as a plasmid, circular vims or circular chromosome) is amplified by polymerase extension of a complementary primer. Up to 10 tandemly repeated, concantemerized copies of the DNA circle are generated by each primer, resulting in one single-stranded, concantemerized product.  

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