Rebinder effect

Insulin and other growth factors result in the phosphorylation of BP-1 at five unique sites. Phosphorylation of BP-1 results in its dissociation from 4E, and it cannot rebind until critical sites are dephosphorylated. The protein kinase responsible has not been identified, but it appears to be different from the one that phos-phorylates 4E. A kinase in the mammalian target of rapamycin (mTOR) pathway, perhaps mTOR itself, is involved. These effects on the activation of 4E explain in part how insuhn causes a marked posttranscriptional... 

Effective ligand rebinding from the Xel site is only observed when the temperature has risen above a characteristic temperature 180 K (Figure 1.6), which is the temperature where protein dynamics sets in. However, the time-related information is lost in experiments at cryogenic temperatures. Time-resolved crystallography was applied to restore the time scale and observe undisturbed relaxations. 

In the early works by Soviet scientists (primarily the followers of P. A. Rebinder and G. V. Vinogradov) and Western investigators (H. C. Booij, R. I. Tanner, J. M. Simmons, T. Kataoka, R. Osaki, et al.) published between 1966 and 1968, the vibration was proven to produce a powerful effect on rheological properties of two-phase disperse systems, filled polymers, rubbers as wellas dissolved and molten polymers. 

The rebinding dynamics to microperoxidase are nonexponential due to a solvent cage effect. To deduce the time constant for rebinding CO from the solvent cage, the recombination kinetics were modeled according to... 

Not only the rigidity is crucial to the efficiency of MIPs, but also the accessibility as many recognition sites as possible should be accessible for rebinding. The material should therefore be porous. This is realised by dissolving monomers, cross-linkers and print molecules in a porogenic solvent prior to polymerisation. The effect of the solvent on the polymer morphology can be monitored by measuring physical parameters such as surface area, pore diameter and pore volume. 

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