Real-time monitoring, protein interaction

Gales, C., Rebois, R. V., Hogue, M., Trieu, P., Breit, A., Hebert, T. E., and Bouvier, M. (2005). Real-time monitoring of receptor and G-protein interactions in living cells. Nat. Methods 2, 177-184. 

Another major advantage of molecular graphics is that it allows real time manipulation of several interacting molecules while quantitatively monitoring the stereochemistry and even the interaction energies. These methods are only beginning to be used for studies of interactions between molecules and only in the most preliminary way for the simulation of protein adsorption 29). 

This phenomenon has been utilised to measure the time course of the interactions of just about any charged molecules such as ions e.g. Ca " ", peptides, and proteins that may interact with either synthetic and biological membranes with great sensitivity in real time. An example of this is shown in Fig. 3b. It has also proved possible to monitor the early events during the interactions of macromolecules with artificial membrane systems (25) and with living cells (12) to obtain thermodynamic information of the intermolecular interactions (13). 

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