Raucaffricine glucosidase

Ruppert M, Panjikar S, Barleben L, Stockigt J. Heterologous expression, purification, crystallization and primary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis. Acta Crystallo-graph Sect F Struct Biol Cryst Commun 2006 62 257-60. 

SG and raucaffricine glucosidase share a high degree of structural similarity. Both are members of the GH-1 superfamily. They are illustrated and compared in the paragraph on raucaffricine glucosidase later in Section 4.3.2. 

The glucoalkaloid raucaffricine has been reported to be synthesized in Rau-volfia cell cultures as the major alkaloid. Isolation and cloning of the cDNA of raucaffricine glucosidase enabled overexpression of the recombinant enzyme which can be prepared in applicable amounts and used efficiently in chemo-enzymatic routes. 

WARZECHA, H., OBITZ, P., STOECKIGT, J., Purification, partial amino acid sequence and structure of the product raucaffricine-0- 3-D-glucosidase from plant cell cultures of Rauwolfia serpentina., Phytochemistry, 1999, 54,657-666. 

Schiibel, H., Stockigt, J., Feicht, R. and Simon, H. (1986) Partial purification and characterisation of raucaffricine fJ-D-glucosidase from plant cell suspension cultures of Rauwolfia serpentina Benth. Helv. Chim. Acta, 69, 538-47. 

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