Rapid scanning stopped-flow design

Me had a number of goals in mind when we set out to design a new vidicon rapid scanning stopped-flow spectrophotometer. 

Before any instrument can be used with confidence its operating characteristics must be well understood. The exceptional versatility of the vidicon rapid scanning stopped-flow system allowed a large variety of characterization experiments to be easily performed. A few of the more important experiments designed to help characterize the vidicon detector will be presented before the actual application of the instrument is discussed. 

Fig. 11. Rapid-scanning, stopped-flow data comparing the accumulation of transient intermediates during the reaction of 8 mM z>i-[a- H]-serine (A) and 8 mM o/.-[a-2H]-serine (B) with 13.3 M a232 from E. coli. The trace designated 0 is the reconstructed spectrum of the reactants before mixing. The insets to both (A) and (B) are 460-nm reaction time courses reconstructed from the RSSF data. Each experiment was conducted using 0.1 M potassium phosphate and 1 mM EDTA buffer at pH 7.80 and25°C. All conditions refer to concentrations immediately after mixing. The initiation of scanning in both (A) and (B) occurred 2 ms after flow stopped. Scans 2 through 19 were collected at 4.7, 9.3, 14.0, 18.7,23.4,28.0,32.7,42.0,51.4,60.7,70.1,107, 154,247,387, 761, 1135, and 1980 ms after the first scan, respectively, with a repetitive scan rate of 4.7 ms/scan. [Taken from Drewe and Dunn (85) with permission.]...

A newly designed, high-resolution rapid-scan-stopped-flow spectrophotometer has been used to study the sequential aquation reactions shown in equation (34), where ki = 86.6 s" k2 = 5.2 s and k = 0.145 s at 298.2 K in... 

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