Ralstonia eutropha Cupriavidus

Staphyloferrin B (59, Fig. 17) is produced together with staphyloferrin A (see below Sect. 4.4) by Staphylococcus hyicus and other staphylococci 94, 131), by Ralstonia eutropha 250) (= Cupriavidus metallidurans 90a)). Comparison of its CD spectrum with those of model compounds suggests the (S)-configuration of the central citric acid C-atom. Mass spectral investigations show a 1 1 Fe V to-ligand ratio, and NMR studies of the Ga " complex confirm the participation of the two ot-hydroxy- and of the a-amino acid functions in complex formation. Uptake studies with Fe " showed that staphyloferrin B acts as a siderophore, but it is less efficient than staphyloferrin A. 

Among these microorganisms, Cupriavidus necator [Ralstonia eutropha), Azotobacter vinelandii and recombinant Escherichia coli are the most popular due to their yield and high biopolymer formation rate. Ralstonia eutropha is now called Cupriavidus necator. Most biodegradable polymers show... 

Intracellular PHB Depolymerase of Cupriavidus necator. Note that C. necator was previously known as Ralstonia eutropha, Wautersia eutropha, Alcaligenes eutropha and Hydrogenomonas eutropha. iPHB depolymerases of PHAscl-accumulating bacteria were not described before the year 2000 but since then not less than seven putative iPHB depolymerases and two 3-hydroxybutyrate oligomer hydrolases have been postulated for C. 

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