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Radioreceptor assays standard curve

Fig. 2. Radioreceptor assay standard curves for GABA. Values shown represent results using two different incubation volumes. Fig. 2. Radioreceptor assay standard curves for GABA. Values shown represent results using two different incubation volumes.
Typical Standard Curve for ( H)-GABA Radioreceptor Assay Inhibition of Specifically Bound ( H)-GABA by Known Concentrations of Unlabeled GABA... [Pg.138]

Since the kinetics of receptor binding, and therefore the sensitivity of a radioreceptor assay, may vary with tissue concentration or incubation conditions, it is prudent to calculate a standard curve for the displacement of radioligand during each experiment. To this end, the amount of radioligand bound in the presence and absence of various concentrations... [Pg.84]


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