Radioimmunoassay of Hydromorphone and Hydrocodone in Human Plasma

Hydromorphone (I) and hydrocodone (II) belong to the morphine group of drugs and are used invariably in combination with other ingredients in a number of proprietory antitussive and analgesic antipyretic mixtures. However, interest in the pharmacokinetics of hydromorphone and hydrocodone in human subjects required an adequate assay for drug levels in plasma. 

RIA for hydromorphone and hydrocodone are fairly sensitive in the nanogram per millilitre range but essentially require the preparation of a specific antibody. The laid-out RIA method is quite capable of estimating the above drugs within a range of 2.5-20 ng ml 1 using standard 100 pi plasma sample only. 

RIA-Procedure The different steps to be followed are stated below, namely  

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IL Honigberg, JT Stewart. Radioimmunoassay of hydromorphone and hydrocodone in human plasma. J Pharm Sci 69 1171, 1980. 

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