Radioactive compounds, analysis synthesis

In complex mixtures of compounds (for exanq>le, in organic synthesis or biochemical systems) it may be quite difficult to ascertain the exact amount of a specific component. A widely used technique of considerable value is isotope dilution analysis. This can be iq)plied either with stable isotopes, in which case the detector is a mass spectrometer, or with radioactive isotopes, using measuring techniques presented in Chapter 8. The use of stable isotopes is usually limited to geologic samples, as described in 2.3, but may be applied to biological samples using highly enriched or However, the technique with... 

There are three known mechanisms for the synthesis of SEs in animal tissue (Fig. 3). It seemed likely that one of these might apply in the spinach leaf. Palmityl-CoA served as a donor, but several compounds were labeled before SE, and these could have served as the actual acyl donor (Garcia and Mudd, 1978a). Radioactive PC was a good acyl donor, but product analysis showed after short times that DG was the predominant material. When DG was isolated from reaction mixtures, it proved to be an excellent acyl donor. Subsequent work with chemically prepared DG has confirmed the sugges-... 

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