Radio-HPLC chromatograms

Fig. 7 Radio-HPLC chromatogram for RP444 and the complex [99mTc(HYNIC-D-Phe)(TPPTS)(tricine)]...

Fig. 9 Radio-HPLC chromatogram for the chirality experiment. The single peak at 12.5 min is due to [99mTc(HYNIC-BA)(tricine)(PSA)], where HYNIC-BA is AT-benzyl-6-(2-sulfobenz-aldehydehydrazono)nicotinamide and PSA is pyridine-3-sulfonic acid, and the peaks at 19.8 and 20.4 min are from [99mTc(HYNIC-MBA)(tricine)(PSA)], where HYNIC-MBA is N-((R) (+)-a-methylbenzyl)-6-(2-sulfobenzaldehydehydrazono)nicotinamide...

Fig. 7 Typical radio-HPLC chromatograms of 90Y-TA138 (top) and mIn-TA138 (bottom)...

Figure 4. Radio-HPLC chromatograms showing accumulation of CoA esters from [U- C]hexadecanoyl-CoA by rat heart mitochondria in the presence of (A) 0,(C) 2mM potassium maleate. Rat heart mitochondria were incubated with 90 xM [U- Clhexadecanoyl-CoA and CoA esters analysed by radio HPLC. The chromatograms are representative of 3-S incubations. Peak identification 1, TCA cycle intermediates, 2, acetyl- 3, dec-2-enoyl- 4, 3-hydroxydodecanoyl- 5, decanoyl- 6, dodec-2-enoyl- 7, 3-hydroxytetradecanoyl- ...

Figure 1. Representative radio-HPLC chromatograms of in vitro metabolism of [ CJmethoxychlor by precision-cut rat liver slices. The chromatograms show the radioactive components detected in the slice extracts after 1-hr incubation and in cultured medium after 4-hr incubation.

Fig. 4 Typical radio-high-performance liquid chromatography (HPLC) chromatograms of macrocyclic 99mTc complexes [99mTc(HYNIC-Kp-DPPB)(tricine)] (top) and [99mTc(HYNIC-Ko-DPPB)(tricine)] (bottom), where HYNIC-Kp-DPPB is iST- -(2-(diphenylphosphino)ben-zoyl)-N-a-(6-(2-(2-sulfonato-benzaldehyde)hydrazono)nicotinyl)lysine methyl ester and HYNIC-Ko-DPPB is iSr- -(4-(diphenylphosphino)benzoyl)-AT-fl-(6-(2-(2-sulfonato-ben-zaldehyde)hydrazono)nicotinyl)lysine methyl ester...

The principal feature which distinguishes the metabolism of allopurinol in leishmania is the rapid formation of allopurinol ribonucleoside monophosphate (HPPR-MP). This can be demonstrated by grow hjL donovani in the presence of (6- C)-allopurinol and analyzing the perchloric acid-soluble extracts by high performance liquid chromatography (HPLC). It is the predominant radioactive material in the elution profile. The chromatogram reveals three other radio-labeled compounds. The first radioactive metabolite, which appears ahead of HPPR-MP, is 4-aminopyrazolopyrimidine ribonucleoside monophosphate (APPR-MP). The second is between ADP and GDP and the third between ATP and GTP. These are 4-aminopyrazolopyrimidine ribonucleoside diphosphate (APPR-DP) and triphosphate (APPR-TP), respectively. When the RNA from these cells is analyzed, 4-aminopyrazolopyrimidine (APP) can be found in the RNA. ... 

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