Rabbit myeloma cell lines

Antigen-specific antibodies produced by B-cells are secreted and appear in the blood serum of an immunized animal, from where they can be isolated as polyclonal mixtures, usually from rabbits. Alternatively, isolation of B-cells from the spleen of an immunized mouse and fusion with myeloma (tumor cells) yields hybridoma , which are antibody-producing cells that can be propagated in vitro [4]. Each hybridoma cell line secreting an antigen-specific antibody can be isolated and provides a source of a single monoclonal antibody. Polyclonal and monoclonal antibodies to a variety of proteins are commercially available, and many industrial and academic laboratories offer to produce polyclonal or monoclonal antibodies. 

---