Rabbit muscle hybrids

Keutel, H.J. Okabe, K. Jacobs, H.K. Ziter, R Maland, L. Kuby, S.A. Studies on adenosine triphosphate transphosphorylases. XL Isolation of the crystalline adenosine triphosphate-creatine transphosphorylases from the muscle and brain of man, calf, and rabbit and a preparation of their enzymatically active hybrids. Arch. Biochem. Biophys., 150, 648-678 (1972)... 

Fig. 10. Hybridization of GAPDH s (a) rabbit (R) and lobster (L) muscle, pig (P) and lobster (L) muscle (b) rabbit (R) and yeast (Y), pig (P) and yeast (Y). The hybrid bands were separated by electrophoresis on cellulose acetate in 50 mM phosphate buffer, pH 7.0, and revealed in (a) by protein staining and in (b) by activity staining (cf 103).

Troponin components of bovine cardiac muscle were separated by essentially the same procedure as for skeletal troponin components (Tsukui and Ebashi, 1973). Studies on the Ca + sensitivity of superprecipitation with various combinations of troponin components from rabbit skeletal and bovine cardiac muscles showed that most hybrid troponins gave the lower Ca sensitivity of superprecipitation (Ebashi, 1974a,b) (Table III). The lowest value was obtained with the combination of skeletal troponin C-T and cardiac troponin I. However, when cardiac troponin T was combined with skeletal troponin I—C, the calcium sensitivity was exceptionally high and exceeded those obtained by the native... 

NPY receptors exist in many central and peripheral tissues crucial for cardiovascular control. Peripheral NPY receptor-types have been identified by various methods. Radioligand binding has demonstrated Y1 receptors in pig renal artery and dog spleen (Lundberg and Modin, 1995), while Y2 receptors have been identified in pig spleen (Lundberg and Modin, 1995) and rabbit kidney (Sheikh etal., 1989). In blood vessels of human kidney, Y1 receptor mRNA has been detected with in situ hybridization (Wharton et al., 1993). Mainly Y1 receptors have been demonstrated in cultured vascular smooth muscle cells from porcine and rat arteries and veins (Mihara et al., 1990 Shigeri et al., 1991 Grundemar et al., 1992). Autoradiographic studies have labelled mainly Y1 receptors in small arteries in, for example, rabbit kidney (Leys et al., 1987) and rat pancreas (Sheikh et al., 1991), and intracardiac arterioles (Allen et al., 1993). On the other hand Y3-like receptors may be present in rat heart (Balasubramaniam et al., 1990). 

---