Quorum sensing inhibition

Inhibition of Quorum Sensing as an Anti-infective Strategy. 307... 

Ramage G, Saville SP, Wickes BE, and Lopez-Ribot JL, Inhibition of Candida albicanshiofAm formation by farnesol, a quorum-sensing mo ecv e, Appl Environ Microbiol 68 5459—5463, 2002. 

Tateda, K., Comte, R., Pechere, J.C., Kohler, T., Yamaguchi, K., Van Delden, C. Azithromycin inhibits quorum sensing in Pseudomonas aeruginosa. Antimicrob. Agents Chemother 2001 45 1930-33. 

Amara N, Mashiach R, Amar D et al (2009) Covalent inhibition of bacterial quorum sensing. J Am Chem Soc 131 10610-10619... 

Ritchie AJ, Jansson A, Stallberg J, Nilsson P, Lysaght P, Cooley MA (2005) The Pseudomonas aeruginosa quorum-sensing molecule N-3-(oxododecanoyl)-L-homoserine lactone inhibits T-cell differentiation and cytokine production by a mechanism involving an early step in T-cell activation. Infect Immun 73 1648... 

Further, compartmentalization allows cells to control the import of nutrients and the export of products that manipulate the environment, or excrete waste. These include siderophores that enhance uptake of metal ions, quorum-sensing molecules that inform cells of the presence of others of their kind, molecules that form biofilms that allow attachment to surfaces and protection from mechanical and chemical stress, and toxins that inhibit the growth of competitors. 

The comQXP loci of a set of natural Bacillus isolates have been sequenced and shown to possess a striking polymorphism that determines specific patterns of both activation and inhibition of the quorum sensing response.217 Genetic and biochemical evidence demonstrate that all the ComX variants are isoprenylated by the posttranslational modification of a conserved tryptophan residue and that the modifications on the ComX peptide backbones vary in mass among the various phenotypes. 

Hentzer M, Riedel K, Rasmussen TB, Heydom A, Andersen JB, Parsek MR, Rice SA, Eberl L, MoUn S, Hoiby N, et al. Inhibition of quorum sensing in Pseudomonas aeruginosa biotilm bacteria by a halogenated furanone compound. Microbiology 2002 148 87-102. 

Choo JH, Rukayadi Y, Hwang JK Inhibition of bacterial quorum sensing by vanilla extract. Lett. Appl. Microbiol. 2006 42 637-641. 

Smith KM, Bu Y, Suga H. Induction and inhibition of Pseudomonas aeruginosa quorum sensing by synthetic autoinducer analogs. Chem. Biol. 2003 10 81-89. 

Staphylococci are responsible for most of the nosocomial infections. Since these bacteria are resistant to several antibiotics, there is an urgent need for novel drugs against Staphylococci infections. Quorum-sensing inhibitors that block the cell-to-cell communication between Staphylococci and thereby inhibit their virulence could represent an alternative to antibiotics. A pharmacophore-based screening retrieved hamamelitannin (7, Figure 5.11) from the bark and leaves of Hamamelis virginiana. 

Detection and Inhibition of N-Acylated Homoserine Lactone Quorum Sensing... 

For testing for the presence of inducers or repressors of AHL-based quorum sensing, the same E. coli monitor strain can be used. To detect inducers, one can use directly the monitor strain, which does not produce its own AHL signal. Whereas this monitor strain works well for most AHL molecules, it is not sensitive to C4-AHLs and another strain must be used, and the method for testing for the C4-AHLS is described elsewhere (19). To test for inhibition of signaling, one must use the monitor strain in the presence of both the test substance and exogenously added AHL, which in this case is the 3-oxo-6C-AHL. 

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