Quantitative Sugar Analysis, Including Sialic Acid

Quantitative Sugar Analysis, Including Sialic Acid (Chambers and Clamp 1971, Clamp et al 1971, Kamerling et al 1975 a) 

The quantitative sugar analysis is carried out on a CPsil5 WCOT fused silica capillary column (25 mx 0.32mm, i.d.) using flame-ionization detection. The carrier gas nitrogen flow-rate is 1.5 ml/min and the make-up gas nitrogen flow-rate 35ml/min. The split-ratio amounts 1 10. The injection-port temperature is 210 °C, and the detector temperature 230 X. The oven temperature is 

Remarks 1. The amount of added internal standard depends on the carbo-hydrate-content in the sialoglycoconjugate 2. When too much acetic anhydride is added, the primary hydroxyl functions of mannitol are 0-acetylated giving rise to an additional small peak in the gas chromatogram. The same holds for the primary hydroxyl function of NeuSAc methyl ester methyl glycoside 3. Molar adjustment factors of monosaccharides except NeuSAc are determined by application of the methanolysis procedure on standard mixtures of sugars and internal standard. For NeuSAc the molar adjustment factor is determined using a 

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