Quantitation, Interpretation and Reporting of Results

It is obviously desirable to be able to defect IgE antibodies in very sensitive, specific, and accurate assays and to report the results in quantitative terms. Over the last two or three decades, a variety of methods for quantification have been developed. Many laboratories have reported results using a class or scale system ranging from class 0, no reaction to class one, a low level of specific IgE and so on to class six, an extremely high level. Although such a semiquantitative scale helps to interpret, more finely discriminate, and sort the relative strengths of flie different 

Drug (or close structural analog) covalently linked to suitable solid phase 

Monospecific, affinity-purified anti-human IgE antibodies tagged with suitable reporter group (e.g., radioisotope, enzyme, fluorescent label, colloidal rtretal particles, etc.) 

Serum from cord blood (IgE- fiee control) 

Sera from subjects allergic to corrrmon allergens include sera with high total IgE levels 

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