Quality Control and Standardization of Caco-2 Assay

By standardizing the following parameters, it is possible to improve the quality of data, as well as to increase the opportunities to compare data produced by different laboratories  

These suggested factors for standardization of the Caco-2 model might also be reasons for the variability in published permeability values obtained from different laboratories [86]. Clearly, data from different publications or laboratories should not be mixed without prior harmonization of the experimental protocols. 

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