Pyrobaculum aerophilum

Lee, S., Sawaya, M. R., and Eisenberg, D. (2003) Structure of superoxide dismutase from Pyrobaculum aerophilum presents a challenging case in molecular replacement with multiple molecules, pseudo-symmetry and twinning. Acta Crystallogr. D 59, 2191-2199. 

Volkl, P., Huber, R., Drobner, E., Rachel, R., Burggraf, S., Trincone, A., and Stetter, K. O. (1993). Pyrobaculum aerophilum sp, nov., a novel nitrate-reducing hyperther-mophilic archaeon. Appl. Environ. Microbiol. 59, 2918-2926. 

Y. M. Drozdowicz, Y. P. Lu, V. Patel, S. Fitz-Gibbon, J. H. Miller and P. A. Rea (1999). A thermostabile vacuolar-type membrane pyrophosphatase from the archaeon Pyrobaculum aerophilum implications for the origins of pyrophosphate-energized pumps. FEES Lett., 460, 505-512. 

Low solubility or improper folding may sometimes hamper the use of enzymes, particularly when expressed in a non-native host. A method of expressing proteins with a C -terminal GFP fusion to use fluorescence as a measure of the amount of correctly folded protein has been introduced (44). DNA shuffling produced variants of ferritin that showed increased solubility, even when they were recloned without the GFP fusion. This assay has been used to produce proteins for X-ray crystallography structure determination (45). The protein nucleoside diphosphate kinase from Pyrobaculum aerophilum is insoluble when expressed in E. coli, but after DNA shuffling, a functional variant with six mutations was found to have 90% solubility, which enabled its crystallization, and its structure was determined. 

Das A, Mishra AK, Roy P (1992) Anaerobic growth on elemental sulfur using dissimilar iron reduction by autotrophic Thiobacillus ferrooxidans. FEMS Microbiol Lett 9 167-172 de Bruijn P, van de Graaf AA, Jetten MSM, Robertson LA (1995) Growth of Nitrosomonas euro-paea on hydroxylamine. FEMS Microbiol Lett 125 179-184 de Jong GAH, Hazeu V, Bos P, Kuenen JG (1997) Isolation of the tetrathionate hydrolase from Thiobacillus acidophilus. Eur J Biochem 243 678-683 de Vries S, Strampraad MJ, Lu S, Moenne-Loccoz P, Schroder I (2003) Purification and characterization of QH2 NO reductase from hyperthermophilic archaeon Pyrobaculum aerophilum. J Biol Chem 278 35861-35868... 

Pyrobaculum aerophilum 2.22 2000 Hypeitheimophile/sewage digestion/ biogas production... 

Los Alamos National Laboratory and The University of California, Los Angeles Thermophilic archeon Pyrobaculum aerophilum http //www-structure. llnl.gov/PA/PA intro.html... 

Mutator features appear first in the archaea. The anaerobic crenarchaeon, Pyrobaculum aerophilum lives in boiling marine water holes. Its mRNA does not express 5 imtranslated regions, therefore its protein translation is initiated without a ribosome binding site ( leaderless transcripts ). It synthesizes carbohydrates through... 

Sakuraba H, Yokono K, Yoneda K, Watanabe A, Asada Y, Satomura T, Yabutani T, Motonaka J, Ohshima T (2010) Catalytic properties and crystal structure of quinoprotein aldose sugar dehydrogenase from hyperthermophilic archaeon Pyrobaculum aerophilum. Arch Biochem Biophys 502(2) 81-88... 

Knott JM (2009) Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum. FEBS Lett 583 3519-3524 Knott JM, ROmer P, Sumper M (2007) Putative spermine synthases from Thalassiosirapseudonana and Arabidopsis thaliana synthesize thermospermine rather than spermine. FEBS Lett 581 3081-3086... 

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