Pyrethrins and Synergists

For separating the pyrethrins and synergists, sifica gel layers (standard method, chamber saturation) and various so-called chloro-form-isoeluotropic solvents have been employed for development 1. benzene-butanone (90+10) 2. benzene-ethyl acetate (85+15) 3. carbon tetrachloride-ethyl acetate (80 + 20) 4. hexane-butanone (80 + 20) 5. hexane-ethyl acetate (75 + 25). 

It must be pointed out nevertheless, that the pyrethrin I spot contains a mixture of pyrethrin I-compounds the pyrethrin II spot 

T standard Desaga mixture B butter yellow / or J indophenol 8 Sudan red) 

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The detection of microgram quantities of pyrethrins, cinerins, keto alcohols, and chrysanthemum acids by paper chromatography and by application of these techniques to a study of possible metabolites enabled certain tentative conclusions that imply hydrolysis in insects of a large portion of the radioactive pyrethrins and synergists to corresponding keto alcohols and chrysanthemum acids. 

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