Pulse Sampling or Gated Detection

While TCSPC andFD fluorometry are the dominant methods used by biochemists, there are alternative methods for measuring intensity decays. Prior to the introduction of 

Gated detection can be accomplished in two ways. One method is to turn on or gate the g n of the detector for a short period during the intensity decay. Surprisingly, thb can be accomplished on a timescale adequate for measurement of nanosecond lifetimes. Alternatively, the detector can be on during the entire decay, and the electric cal pulse measured with a sampling oscilloscope. Such devices can sample electrical signals with a resolution of tens of picoseconds. 

In recent years there has been a reintroduction of g ed detection methods. The time resolution can be good but is not con arable to that of a laser source and an MCT PMT. Typical instrument response functions are close to 3 

---