Pulse-field gel electrophoresis PFGE

Use of pulsed-field gel electrophoresis (PFGE) to separate large DNA fragments Permits isolation of large DNA fragments obtained by use of restriction endonucleases (rare cutters) that result in very limited cutting of DNA. 

PCR is now the front-end process for other DNA profiling techniques such as pulse-field gel electrophoresis (PFGE), terminal restriction... 

Figure 4.8 Patterns of smaldigested DNA of Staphylococcus aureus isolates on pulsed-field gel electrophoresis (PFGE). The isolates were obtained from four patients in diversely distant locations within the United States. Lane 1 = ATCC29213 (positive control), lane 2 = a methi-cillin-resistant S. aureus isolated from a patient in Georgia lane 3 = a gylcopeptide intermediate S. aureus isolated from a patient in Michigan, lane 4 = a methicillin-resistant S. aureus from the same patient in Michigan, and lane 5 = a glycopeptidet-intermediate S. aureus isolate from a patient in New Jersey. (From Smith et al., 1999b.)...

Fig. 2 Viral genome sizes of different Phaeocystis globosa virus isolates (PgV) determined by pulsed-field gel electrophoresis (PFGE). Lane M Lambda concatamers ladder, Lane 1 uninfected culture of P. globosa, Lane 2 PgV-04 (genome size of 175 kb), Lane 3 PgV-12 T (genome size of 465 kb). The small-sized band (approximately 45 kb) as seen in lanes 1-3 correspond to bacteriophages since algal cultures were not axenic...

Pulsed Field Gel Electrophoresis (PFGE) Isoelectric Focusing of Proteins 1 27... 

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