Pseudomonas pyocyaneus

TUlandsia usenoides. An antibacterial substance (176) was extracted from the brown parts of Spanish moss with acetone or chloroform. It was active against S. aureus, Pneumococcus I, II, and III, S. hemolyticus C 203, Hemophilus influenzas type B, and Candida albicans. It was not active against E. coli. Pseudomonas pyocyaneus, and H. influenzae type A. 

Pyocyanine. 1 -Hydroxy-S-methylphenazini u m hydroxide inner salt. C,3HWNJ3 mol wt 210.23. C 74.27%, H 4.80%, N 13.32%, O 7.6]%. Produced by Pseudomonas aeruginosa (Ps. pyocyanea, Bacillus pyocyaneus). Isoln from broth cultures Wrede, Si rack. Z. Physiol Chem. 140, 1 (1924). Isoln from agar cultures Sohoental, Brit J, Exp Pathol. 22, 137 (1941). Synthesis by dimethyl sulfate meth-ylation of a -hydroxyphenazine and alkaline treatment of the resulting methosulfate Wrede, Strack, Z. Physio/, Chem. 181, 58 (1929) Ber. 62, 2051 (1929) Surrey, Org- Syn. 26, 86 (1946) coll, voL HI, 753 (1955). 

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