Pseudomonas putida MTCC

Resmi et al. [59] used laterite stones for the immobilization of Pseudomonas putida (MTCC 1194). The amount of bacterial biomass attached to the support was 8.64 g/100 g of stones on dry weight basis. Packed bed reactor was used for treating mixture of seven azo dyes. With the help of immobilized bacterial strain, dye mixture was degraded to nontoxic smaller molecules. It was reported that even after 2 months, bacteria-coated pebbles were stable and suitable for the aerobic degradation of azo dyes. With the help of TLC and HPLC, 61.7% degradation was reported at the concentration of 50 pg/mL of dye. 

Several of the compounds synthesized showed antibacterial activity against E. coli (MTCC 41), S. aureus (MTCC 1144) and the ampicillin-resistant strain Pseudomonas putida (MTCC 1072) (Table 10). 

Naik SC, Kaul P, Barse B, Banerjee A, Banerjee UC. Studies on the production of enanti-oselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110 [J]. Bioresour Technol 2008,99 (1) 26-31. 

General procedure Dynamic kinetic resolution for the synthesis of (R)-mandelic acid 14 In 10 mL of Tris-HCl buffer (0.1 M, pH 7), immobilized or free cells of Pseudomonas putida MTCC 5110 (harboring a stereoselective nitrilase) at 50mg/mL and racemic mandelonitrile 2 (15 mM) were introduced. The solution was stirred for 4 hours at 35 and then stopped by addition of 1-N HCl (0.3 mL). The enzyme was filtered off and the filtrate neutralized to pH 8.5 with 2-N NaOH, washed with diethylether, readjusted to pH 1.5 with 6-N HCl, extracted with diethylether, and finally concentrated. Yield (free) %%, (immobilized cells) 93% ee 98.8%. 

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See also in sourсe #XX -- [ ]

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