Pseudomonas putida-arvilla

Engesser KH, RB Cain, HI Knackmuss (1988b) Bacterial metabolism of side chain fluorinated aromatics cometabolism of 3-trifluoromethyl (TFM)-benzoate by Pseudomonas putida (arvilla) mt-2 and Rhodo-coccus rubropertinctus 1 651. Arch Microbiol 149 188-197. 

Worsey, M. J. Williams, P. A. (1975). Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2 evidence for a new function of the TOL plasmid. Journal of Bacteriology, 124, 7-13. 

The same group has also isolated a strain of Pseudomonas putida-arvilla (PL-strain) from limonene and (+)-a-pinene as the sole carbon source that was capable of growing on (+)-limonene, (+)-a-pinene, (-)-a-pinene, / -pinene, 1-p-menthene, 3-p-menthene and p-cymene as substrates [75]. Limonene was degraded to perillyl alcohol, perillaldehyde and perillic acid. 

FIGURE 14.156 Biotransformation of (-)-P-pinene ( ) hyAspergillus nigerNClM 612 and Pseudomonas putida-arvilla (PL strain). (Modified from Bhattacharyya, P.K. and K. Ganapathy, 1965. Indian J. Biochem., 2 137-145 Rama Devi, J. and P.K. Bhattacharyya, 1978. /. Indian Chem. Soc., 55 1131-1137.)... 

The relative activities were determined in the different ways. Values are given relative to catechol for 1,2-CTD and relative to protocatechuic acid for 3,4-PCD. P.p Pseudomonas putida, P.a Pseudomonas arvilla, Ps Pseudomonas B13,. c) [19]. [18]. 

Table 3 summarizes properties of intradiol-cleaving dioxygenases. 1,2-CTD was first isolated and purified from Pseudomonas fluorescens [3] and characterized as a dioxygenase by Hayaishi et al. [1] (2 g atoms of iron/mol, Mr 80 kDa). 1,2-CTD has been purified from Pseudomonas fluorescens by Nakazawa et al. (2 g atoms of iron/mol, Mr 100 kDa) [5], from Pseudomonas arvilla C-1 by Kojima et al. (2 g atoms of iron (ferric)/mol, Mr 90 kDa) [4] and by Nakai et al. (1 g atom of iron/mol, Mr 63 kDa, aP subunits), from Brevibacterium fuscum p-13 (1 g atom of iron/mol) [23], and from Pseudomonas putida mt-2 (1.1 g of iron/mol, Mr 65 kDa, aa subunits) [19]. Nakai et al. found that three isozymes of 1,2-CTD exist in Pseudomonas arvilla C-1 and are formed by combinations of two nonidentical subunits as dimers, aa, aP, PP [20]. Amino acid composition and sequence of 1,2-CTD were analyzed by Nakai et al. [8,19, 20]. 

The extradiol dioxygenases are more common than the intradiol enzymes, and are more diverse in their reactions.1379 Examples are catechol 2,3-dioxygenase (from Pseudomonas arvilla and Ps. putida) and protocatechuate 4,5-dioxygenase from Ps. testosterone These are colourless and... 

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