Pseudomonas chloroaphis

Oinuma K-I, Y Hashrmoto, K Konishi, M Goda, T Noguchi, H Higashibata, M Kobayashi (2003) Novel aldoxrme dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chloroaphis B23. J Biol Chem 278 29600-29608. 

Another patent by Fujisawa disclosed [31] the structure of FR-901375 from an extract of Pseudomonas chloroaphis No. 2522. While it is a likely H DAC inhibitor, no data have been reported in this regard and the decision seems to have been made to promote FK228 instead as the clinical candidate. In 2001, additional depsipeptide natural products, the spiruchostatins, were reported [32] by Shin-ya s group at the University of Tokyo and Yamanouchi Pharmaceuticals. These compounds were isolated from an extract of Pseudomonas sp. Q71576, on the basis of the ability to increase expression of luciferase driven by the plasminogen activator inhibitor (PAI-1) promoter. Given the structural similarity to FK228, the spiruchostatins were likely to be HDAC inhibitors and this was confirmed in a later patent [33] and in our biochemical studies (see below) with the natural product prepared by total synthesis. 

PMSF, as a sulphonic acid fluoride very reactive by nature, is known as specific trypsin and chymotrypsin inhibitor (Fahrney and Grold, 1963). Howard et al. (1999) have found that Pseudomonas chloroaphis is able to degrade and utilize a polyester-urethane as a sole carbon and energy source using extracellular enzymes. A polyurethane-esterase was isolated molecular weight 27000 daltons. PMSF was suited as an effective polyurethane-esterase inhibitor. 

Howard, G. T., Ruiz, C. and Hilliard, N. P., 1999. Growth of Pseudomonas chloroaphis on a polyester-urethane and the purification of a polyurethanase-esterase enzyme. International Biodeterioration <6 Biodegradation 43, 7 12. 

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