Proximity ligation assay

Using RNA Aptamers and the Proximity Ligation Assay for the Detection of Cell Surface Antigens... 

Gustafedottir, S. M., Schallmeiner, E., Fredriksson, S., Gullberg, M., Soderberg, O., Jarvius, M., Jarvius, J., Howell, M., and Landegren, U. (2005) Proximity ligation assays for sensitive and specific protein analyses. Anal Biochem. 345,2-9... 

Schallmeiner, E., Oksanen, E., Ericsson, O., Spangberg, L., Eriksson, S., Stenman, U. H., Pettersson, K., and Landegren, U. (2007) Sensitive protein detection via triple-binder proximity ligation assays. Nat Methods. 4, 135-137... 

Pai, S., Ellington, A. D., and Levy, M. (2005) Proximity ligation assays with peptide conjugate burrs for the sensitive detection of spores. Nucleic Acids Res. 33, el62... 

In solid-phase proximity ligation assay, low-femtomolar concentrations of the protein were detected using the same pair of proximity probes as those used for the homogeneous assay. This demonstrated the high sensitivity of the detection method compared with the low-picomolar detection threshold of the ELISA for the same protein. 

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