Protonation cont

Table 5. Proton-donating units and the corresponding polymers in Sects. 4-6 (cont.)...

P13 acts in the fission yeast cell division cycle. Addition of the proton to the kinase assay in vitro was able to rescue the defect in the Cdc2 mutant kinase activity P13 cont ns two tryptophans (Trp-71 and Trp-82) and seven tyrosine residues. The maximum of the fluorescence emission spectrum b localcd at 336 nm (>., 295 or 275 nm). Thus, tryptophan residues are responsiUe for the protdn fluorescence. Three lifetimes are found for the intensity dec, 0.6,2.9 and 6.1 ns, with fracUonal intensities of0.04,0.32 and 0.64, respectively. 

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