Proton partial

In detail, 6 nM DNA was incubated for 20 min at 37 C with the restriction enzyme (0.08 units/ jjlI) and a varying concentration of oligomer in the restriction buffer (but at pH 6.5 to maintain the cytosine protonated). Partial protection was obtained by adding 10 jiM oligomer, while at 100 xM protection was virtually complete (50% protection at 20 jxM). Protection was poor at pH 7.9. The use of purine oligomers should relax the requirement of acidic conditions. 

Zeolite Zeolite electronegativity Acid strength Proton partial charge H 0... 

Reaction for acid centre formation Cation electronegativity H o Acid strength Proton partial charge... 

Tbp.-The electrons in the 0—H bonds of H2O molecules are attracted to the oxygen atoms, leaving the positively charged protons partially exposed. A proton on one water molecule is attracted to a lone pair on an oxygen atom in another water molecule.Soffom Lewis structures of water with hydrogen bonding between molecules. 

Since incomplete deuterium incorporation into the phenylcyclo-propane product (29% di) excludes a free carbonium (or diazonium) ion as its precursor, a partially-protonated (partially exchanged) diazo species (20) was proposed to react with olefin to give a hexacoordinate, carbenoid-type intermediate (21) which gives either cyclopropane or syn ion pair (22). Intermediate (20), although not a normal diazonium ion, is presumably electrophilic and should have diazonium ion character, in support of the biogenetic analogy. Also of interest in the above reaction is the product resulting from the combination of olefin alkylation and... 

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