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Proteomic platforms

Proteomic platforms combine separation and identification technologies that can be completed as an experiment in a timely fashion. Proteomic platforms strive to accomplish five critical objectives (1) a high level of information about one or more protein attributes (2) a large number of samples per analysis session (sample throughput) (3) quantitative and comparative protein expression among samples (4) timely analysis of raw and processed data and (5) use of a discovery-oriented or open platform system. [Pg.52]

Balgley BM, Wang W, Song T, et al. Evaluation of confidence and reproducibility in quantitative proteomics performed by a capillary isoelectric focusing-based proteomic platform coupled with a spectral counting approach. Electrophoresis 2008 29 3047-3054. [Pg.365]

Aleo MD, Navetta KA, Emeigh Hart SG et al. (2002) Mechanism-based urinary biomarkers of aminoglycoside-induced phospholipidosis. Comp Clin Pathol 11 193-194 Aleo MD, Navetta KA, Emeigh Hart SG et al. (2003) Mechanism-based urinary biomarkers of renal phospholipidosis and injury. Toxicol Sci 72 (Suppl) 243 Bandara LR, Kennedy S (2002) Toxicoproteomics - a new pre-clinical tool. Drug Disc Today 7 411—418 Chapman K (2002) The ProteinChip biomarker system from Ci-phergen Biosystems a novel proteomics platform for rapid biomarker discovery and validation. Biochem Soci Trans 30 82-87... [Pg.120]

For toxicoproteomics, the ideal proteomics platform would be one that is ... [Pg.871]

All proteomic platforms generally involve separation and identification of proteins. Toxicoproteomics Proteomics applied to toxicology with the aims of identifying critical proteins and pathways affected by chemical and environmental exposures. [Pg.42]

TABLE 4.4. Proteomic Platforms Using Stable Isotopes... [Pg.56]

Stable isotope proteomic platforms Peptides carrying different stable isotopes can be distinguished by high-resolution mass spectrometry, lamu can be resolved. ICAT, iTRAQ, and SILAC are examples. [Pg.56]

Figure 4.13. Summary of advantages and limitations of representative proteomic platforms for protein expression analysis. The capabilities and advantages (solid line) are compared to the drawbacks (dotted lines) for each proteomic platform. Explanation of advantages and limitations are briefly highlighted here and more thoroughly discussed in the text. Abbreviations for each platform are provided in the Figure 4.1 legend and in the text. Other abbreviations used are PTMs, posttranslational modifications ID, identification. Figure 4.13. Summary of advantages and limitations of representative proteomic platforms for protein expression analysis. The capabilities and advantages (solid line) are compared to the drawbacks (dotted lines) for each proteomic platform. Explanation of advantages and limitations are briefly highlighted here and more thoroughly discussed in the text. Abbreviations for each platform are provided in the Figure 4.1 legend and in the text. Other abbreviations used are PTMs, posttranslational modifications ID, identification.
The top tier of proteomic analysis categories—proteomic mapping and proteomic profiling—will continue to dominate the field of proteomics because investigators are interested in what proteins are present in their sample and how much. How much is typically a proportion of experimental treatment to control, but proteomic technologies are improving on their abilities to provide amounts and concentrations. Therefore, an important consideration in proteomic analysis is a realistic sense of how much of the proteome can actually be measured with the proteomic platform available in order to best answer the scientific problem at hand. [Pg.65]

Synaptic Fraction Source Proteomic Platform Proteome Analyzed Reference... [Pg.83]

A previously published review on the role of proteomics in toxicology [74] covered the earlier toxicoproteomics literature, so the review in Table 5.1 will cover more recent citations with minimal overlap. Table 5.2 summarizes data from primary literature that reports on the effects of toxic agents on global protein expression. Entries are summarized by author, proteomic platform, chemicals or toxicants tested, the tissue analyzed, and a very brief statement of results and potential markers of toxic effect. [Pg.110]

The preceding studies suggest that some of the variability in correlating protein and transcript levels involve (1) sampling time after treatments (mRNA changes precede protein expression), (2) the high information density of DNA arrays and proteomic platform capacity for identified proteins (many transcripts compared to few proteins), (3) very different bioinformatic methods... [Pg.117]

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Advantages and Disadvantages of Proteomic Platforms

Proteomic platforms, advantages

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