Proteomic platforms, advantages

Figure 4.13. Summary of advantages and limitations of representative proteomic platforms for protein expression analysis. The capabilities and advantages (solid line) are compared to the drawbacks (dotted lines) for each proteomic platform. Explanation of advantages and limitations are briefly highlighted here and more thoroughly discussed in the text. Abbreviations for each platform are provided in the Figure 4.1 legend and in the text. Other abbreviations used are PTMs, posttranslational modifications ID, identification.

The many attributes of proteins mentioned in Table 4.2 make complete global analysis of proteins from cells, tissues, organs, or organisms a formidable analytical challenge. A primary aim of proteomic analysis is to provide researchers with as much data as possible about one or more protein attributes. Flowever, to many researchers, proteomic analysis usually involves the three attributes of identification and quantitation of all proteins in a defined sample space. Only the highlights of each platform will be summarized here. A critical appraisal of capabilities, advantages, and drawbacks are summarized in Figure 4.13 (Section 4.5.6). 

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