Proteolytic agent

Resorcinol is a phenol derivative and its proteolytic power is about 1/40 of that of phenol. It breaks the weak hydrogen links of keratin and acts as a proteolytic agent, even at concentrations as low as 5%. At higher concentrations, it can also precipitate skin proteins and become a protein coagulant. 

Proteolysis during maturation is essential in most cheese varieties. The extent of proteolysis varies from very limited (e.g.. Mozzarella) to very extensive (e.g.. Blue mould varieties) and the products range in size from large polypeptides, comparable in size to intact caseins, through a range of medium and small peptides to free amino acids. Clearly, no one proteolytic agent is responsible for such a wide range of products. 

M.M. Krasnov, G.S. Polunin, Impact of certain proteolytic agents on the... 

Mucolytics reduce the viscosity of tenacious and purulent mucus, thus faciUtating removal. The distinction between mucolytics and other classes of expectorants is frequently blurred. Steam, sometimes in conjunction with surfactants or volatile oils, has long been used to decrease viscosity by physical hydration. However, agents that chemically depolymerize certain components of mucus are available. Trypsin and other proteolytic enzymes have shown good clinical activity because of their abiUty to cleave glycoproteins. Pancreatic domase, which depolymerizes DNA found in purulent mucus, also has shown clinical utihty. 

Proteolytic enzyme from the latex of Carica papaya with an approximate molecular weight of 27000. It is differentiated from papain in electrophoresis behavior, in solubility and in substrate specifity. Isolation by acidify of papaya-latex with HCl, salting out with NaCl and following chromatographic purification. The formulation contains L-cysteine as reducing agent. 

Rhinoviri are the causal agents of common colds in humans. Viral replication and maturation is dependent on proteolytic processing of a viral polyprotein by a cysteine protease known as 3C protease. The active-site cysteine in 3C protease... 

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

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