Proteo liposomes

The phospholipid concentration in a (proteo)liposome suspension can be determined by phosphorus analysis (73) and the protein concentration by automated amino acid analysis or by a calibrated colorimetric protein assay... 

Thin-layer chromatography (75) and sodium dodecylsulfate-(poly)acrylamide gel electrophoresis (SDS-PAGE) are helpful for analyses of the lipid and protein composition, respectively. Size-exclusion chromatography allows estimation of the size distribution of the (proteo)liposomes and crude fractionation of the material as reviewed in Ref. 76. Accurate determinations of size distributions require analyses by static or dynamic... 

The incorporation of purified Na /D-glucose cotransporter into the BLM formed by the monolayer folding method was achieved either by fusion of its proteo-liposomes or by folding the lipid layer containing the proteoliposomes. The experimental setup for measuring the currents is the same as in Figure 25. The concentration dependence of the observed currents is shown in Figure 31. A Na ... 

Levy, D., Seigneuret, M., Bluzat, A., Rigaud, J.-L. (1990). Evidence for proton countertransport by the sarcoplasmic reticulum Ca2+-ATPase during calcium transport in reconstituted proteo-liposomes with low ionic permeability. J. Biol. Chem. 265,19524-19534. 

Tajib Mirzabekov, Hairy Kontos, Michael Farzan, Wayne Maiasco and Joseph Sodroski, Paramagnetic proteo-liposomes containing a pure, native and oriented seven-membrane segment protein, CCR5, Nature Biotechnology, 18 (2000), 649-654. 

The second aspect of the mitochondrial process is ATP synthesis. As proposed by Mitchell, it establishes a proton and/or potential gradient which is used to drive ATP synthesis from ADP and phosphate ions. Packer and Stoeckenius reported photophosphorylation in reconstituted proteo-liposomes from a mixture of bacteriorhodopsin, beef-heart ATPase, and soybean phospholipids (see Oosawa and Imai ). This outstanding, often cited experiment does not appear to have been repeated by others. It would be, therefore, of interest to test the chemiosmotic hypothesis using planar BLM. In the BLH system, both pH and applied potential gradients can be easily created. 

ELECTRIC POTENTIAL AND pH GRADIENT FORMATION BY RECONSTITUTED ATPase PROTEO-LIPOSOMES FROM THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS 6716... 

The effect of temperature on ATP dependent 9AA fluorescence quenching in CFq-CFi proteo-liposomes. 

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