Proteins varietal identification

Another major use of computers for HPLC data should be varietal identification (see later). Qualitatively, cereal storage proteins vary little within genotypes but significantly among different varieties, so they provide characteristic fingerprints. Varietal identification can be automated by computer comparisons with stored standard data. Scanlon et al. [80] showed that normalization of peak retention times provided sufficient precision for cultivar identification. Resulting data based on peak heights and times could be used in an automated library search to identify wheat varieties [81]. 

Several studies used RP-HPLC of rice prolamin and glutelin storage proteins for varietal identification [44,123-126], Patterns are genotype specific and little influenced by environment. Long-, medium-, and short-grained cultivars are differentiated, as are closely related sister lines. RP-HPLC identification of rice varieties was reviewed by Lookhart and Juliano [127],... 

Marchylo and Kruger [26,52] developed and optimized conditions to separate barley and malt prolamins (hordeins) by RP-HPLC, and used these methods for cultivar identification based on qualitative and quantitative differences. Elution profiles were largely independent of growth location, year, and protein content. Identification of barley cultivars by hordein lE-HPLC was also reported [164], Barley HPLC varietal identification was reviewed by Marchylo [165],... 

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