Protein microheterogeneous

Although protein microheterogeneity usually disrupts crystal formation, it can occasionally promote crystallization. For example, crystals of Escherichia coli single-stranded binding protein contain a 1 1 mixture of intact and proteolyzed protein (Ollis et ai, 1983). While crystallization experiments were initially conducted with intact protein, crystals grew only when enough molecules to form the mixed crystals had been cleaved by contaminating proteases. 

Some aspects of protein microheterogeneity in relation to their de-naturation behavior will be examined in more detail later on. 

A biologically formed protein or polynucleotide, while exhibiting some limited heterogeneity in length or microheterogeneity in the main chain sequence or in pendant prosthetic groups, has an essentially unique sequence. Different forms of a protein or polynucleotide often can be... 

Hiraoka, A., et al (1998). Sodium dodecyl sulfate-capillary gel electrophoretic analysis of molecular mass microheterogeneity of beta-trace protein in cerebrospinal fluid from patients with central nervous system diseases./. Chromatogr. A 802, 143-8. 

Hiraoka, A., et al (2001). Charge microheterogeneity of the beta-trace proteins... 

When a system contains a fluorophore in different environments (e.g. a fluo-rophore embedded in microheterogeneous materials such as sol-gel matrices, polymers, etc.) or more than one fluorophore (e.g. different tryptophanyl residues of a protein), the preceding relations must be modified. If dynamic quenching is predominant, the Stem-Volmer relation should be rewritten as... 

Fig. 3. Amino acid sequence of several somatic human histone HI proteins to illustrate the microheterogeneity of linker histones. The sequences for human histone HI variants (H1.1-H1.4) were obtained from Ref [373] and Hl-5 was from Ref [412]. The nomenclature followed for the designation of these histone variants was Doenecke (e.g., see Ref. [412]). The nomenclature of Parseghian et at. [373] is shown in parentheses. The regions corresponding to the trypsin-resistant (winged helix motif [96]) which is characteristic of the protein members of the histone HI family are indicated by a boxed inset.

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