Proteins, introduction digestive enzymes

Walters Options The Alternative Cancer Therapy Book (1993) contains a short introduction to the subject under the section Metabolic Therapies, with the statement that proteolytic (protein-digesting) enzymes are believed to dissolve the walls of cancer cells, and that enzyme treatments are widely used in Europe. More specifically, enzymes are used variously in what are called Issels therapy, in Wheatgrass therapy, in the Gerson diet, in Kelley s therapy, and in Niepa- s therapy (Walters, 1993, pp. 86,147,198,207,216). It is noted furthermore that chranotherapy destroys the body s enzymes, that cooking destroys the enzymes in food, and that enzymes may be involved in the so-called spontaneous remission of cancer (Walters, 1993, pp. 155,201). Also noted, in the work of Hans Niepa-, is that the enzyme bromelain, derived from pineapple roots, will deshield cancer cells, as will beta-carotene (Walters, 1993, p. 222). 

Most often proteins are the bacterial biopolymers studied using MALDI MS either from fractions or whole cells. They are not the only isolated cellular biopolymers studied by MALDI, nor the first. Very soon after the introduction of MALDI there were a few reports of the analysis of bacterial RNA or DNA from bacterial fractions. One of the first applications of MALDI to bacteria fractions involved analysis of RNA isolated from E. coli,4 Other studies included analysis of PCR-amplified DNA,5 6 DNA related to repair mechanisms7 and posttranscriptional modification of bacterial RNA.8 While most MALDI studies involve the use of UV lasers, IR MALDI has been reported for the analysis of double stranded DNA from restriction enzyme digested DNA plasmids, also isolated from E. coli.9... 

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