Protein synthesis sensor array

In addition to these organic syntheses, biochemical production of compounds in microreactors has also been performed. A microreactor array which enables high-throughput cell-free protein synthesis was developed [4]. The microreactor array is composed of a temperature control chip and a reaction chamber chip. The temperature control chip is a glass-made chip on which temperature control devices, heaters, and temperature sensors are fabricated with an indium tin oxide (ITO) resistive material. The reaction chamber chip is fabricated by micromolding of polydimethylsiloxane (PDMS)... 

A novel concept for toxin detection is presented based on toxin s inhibition of biological protein synthesis. We demonstrated the feasibility of the concept by confirming differential inhibitory effects of two toxin simulants on the expression yields of three proteins. The unique response pattern is obtained from the 3 X 4 IVT sensor array for each toxin simulant. 

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