Protein stabilized disjoining pressure

T.D. Dimitrova, F. Leal-Calderon, T.D. Gurkov, and B. Campbell Disjoining Pressure vs. Thickness Isotherms of Thin Emulsion Films Stabilized by Proteins. Langmuir 17, 8069 (2001). 

G. Narsimham Maximum Disjoining Pressure in Protein-Stabilized Concentrated Oil-in-Water Emulsions. Colloid Surfaces 62, 31 (1992). 

The results on formation and stability of black foam films, on the first place those on bilayer foam films (NBF) (see Sections 3.4.1.2 and 3.4.4) have promoted the development of methods which enable lung maturity evaluation. The research on stability of amphiphile bilayers and probability for their observation in the grey foam films laid the grounds of the method for assessment of foetal lung maturity created by Exerowa et al. [20,24]. Cordova et al. [25] named it Exerowa Black Film Method. It involves formation of films from amniotic fluid to which 47% ethanol and 7-10 2 mol dm 3 NaCl are added [20,24]. In the presence of alcohol the surface tension of the solution is 29 mN m 1 and the adsorption of proteins from the amniotic fluid at the solution/air interface is suppressed, while that of phospholipids predominates. On introducing alcohol, the CMC increases [26], so that the phospholipids are present also as monomers in the solution. The electrolyte reduces the electrostatic disjoining pressure thus providing formation of black foam lipid films (see Sections 3.4.1.2 and 3.4.4). 

Equation 5.238 was found to fit experimental data well for the disjoining pressure of liquid films stabilized by adsorbed protein molecules bovine serum albumin (BSA)." ° In that case, F was identified with the surface density of the loose secondary protein adsorption layer, while X turned out to be about the size of the BSA molecule." ... 

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