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Protein sorting, subcellular localization

PROTEIN SORTING SIGNALS AND PREDICTION OF SUBCELLULAR LOCALIZATION... [Pg.277]

Nakai, K., and Horton, P. (1999). PSORT a program for detecting the sorting signals of proteins and predicting their subcellular localization. Trends Biochem. Sci. 24,34—35. [Pg.339]

In this chapter, we provide protocols to determine the ability of a peptide to mediate DNA internalization in cultured human tumor cells. Fluorescence-assisted cell sorting (FACS) analysis is used to obtain quantitative data on the time and temperature dependence of macromolecular delivery. Confocal microscopy is used to study the subcellular localization in both fixed and live cells. Fluorescently labeled transferrin and dextran are used to label the clathrin-dependent (15) and the non-clathrin, non-caveolar (16) endocytic compartments, respectively. Expression of a caveolin-l-YFP fusion protein is used to label cell surface caveolae and intracellular caveosomes (17). Finally a protocol, for the overexpression of dominant-negative dynamin [GTPase deficient dynamin-2 containing the amino acid substitution K44A (18)] is provided to evaluate the dynamin dependence of the uptake mechanism. [Pg.102]

Nakai, K. (2000) Protein sorting signals and prediction of subcellular localization. Adv. Protein Chem. 54,... [Pg.275]

See other pages where Protein sorting, subcellular localization is mentioned: [Pg.278]    [Pg.289]    [Pg.331]    [Pg.74]    [Pg.69]    [Pg.385]    [Pg.264]    [Pg.265]    [Pg.266]    [Pg.397]    [Pg.61]    [Pg.412]    [Pg.274]    [Pg.277]    [Pg.171]   


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