Protein separation from biological broths

Affmity separation, advantages for protein and peptide separation from biological broths and blood, 297 Aging, proteins, 247-248 Alkylene oxalate polymers, application, 202 Amine-derivatized poly(ethylene terephthal-ate), preparation, 139,141/... 

After biological reactions have generated a product of interest, it is necessary to recover this product from a liquid mixture that typically contains several undesirable components. The treatment of any culture broth after bioreactor cultivation is known as downstream processing. Downstream processing can account for 60-80%) of the total production cost, particularly in the production of modern recombinant proteins and monoclonal antibodies. A typical downstream process requires several steps in the areas of solid-liquid separation, cell rupture, product recovery, and product purification. It is important to minimize the number of downstream processing steps required because significant product losses inevitably occur during each step.f ... 

Various methods are available for the separation of biochemicals. These include physical methods of centrifugation and filtration, chemical methods of precipitation and extraction, and interactive techniques, such as electrophoresis and chromatography. These methods are employed to perform the steps necessary to purify biological materials from complex solutions. The isolation of a specific component (e.g. a protein) from a plasma source or a fermentation broth requires several steps ... 

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