Protein relation between electrophoretic

The charge (negative or positive net charge) of, e. g. a protein particle, is closely related to its electrophoretic mobility. Approximate information about the likely direction and magnitude of mobility of particles migrating in an electric field, can be obtained from titration results. There is, however, no quantitative agreement between the net charge as calculated from titration curves and from electrophoretic mobility. 

When A. brasilense was cultured in Luria-Bertani rich medium, the surface composition varied during growth, as illustrated by Fig. 40. Modeling the composition in terms of molecular compounds indicated that the protein concentration increased, from 30 (exponential phase cells) to 50% (stationary phase cells), concomitantly with a decrease in the polysaccharide concentration, from 60 to 35%. These modifications were related to a change in cell-surface hydrophobicity, the water contact angle measured on cell lawns increasing from 20 to 60° (Fig. 40). No difference of electrophoretic mobility was detected between cells harvested in the exponential... 

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