Protein purification technolog

In principle, the whole spectrum of protein purification technologies can be applied to antibodies. The best established fall into the following categories ... 

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Hedhammar, M., Graslund, T., and Hober, S. 2005. Protein engineering strategies for selective protein purification. Chemical Engineering and Technology 28, 1315-1325. 

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First, a general method of transformation of the expression plasmid into E. coli will be described, as well as appropriate conditions for growth and induction of the expression cultures. Then, the 96-well protein purification method is detailed. Last, analysis of the purified proteins is described using both lab-on-a-chip technology and traditional sodium dodecyl sulfide (SDS)-polyacryla-mide gel electrophoresis (PAGE). 

HPLC techniques have occupied a dominant position for over two decades in peptide and protein chemistry, in molecular chemistry, and in biotechnology. These techniques with their various selectivity modes (listed later) can be considered the bridges that link cellular and molecular biology (viz., structural proteomics and atomic biology) and industrial process development associated with the recovery and purification technologies that turn these opportunities into realities. Different dominant interactive modes of HPLC are as follows ... 

Purification can also be achieved in other ways, through precipitation with salts, crystallization, and through aqueous two-phase extraction. Some of these methods are associated with substantial capital cost, low throughput, low yields, or waste issues. Fractional precipitation, one of the oldest protein separation technologies, can be surprisingly effective to separate a compound of interest from a complex broth. For instance, the process of fractional solvent precipitation of blood plasma components has been used since World War II. 

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